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rabbit anti human pdk 1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti human pdk 1
    Rabbit Anti Human Pdk 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+pdk+1/PDK1+Antibody/pm36838927-248-63-69
    Average 96 stars, based on 987 article reviews
    rabbit anti human pdk 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: A Meroterpenoid from Tibetan Medicine Induces Lung Cancer Cells Apoptosis through ROS-Mediated Inactivation of the AKT Pathway
    Article Snippet: An aliquot of the denatured supernatant containing 40 mg of protein was resolved on SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with a blocking buffer (Tris-buffered saline, i.e., TBS, containing 5% non-fat milk) for 1 h at room temperature, the membrane was probed with primary antibodies for mouse anti-human caspase-8 (BD Biosciences, San Jose, CA, USA), rabbit anti-human caspase-3, rabbit anti-human poly (ADP-ribose) polymerase (PARP), rabbit anti-human phospho-AKT (Ser473), rabbit anti-human caspase-9, rabbit anti-human AKT, rabbit anti-human PDK-1, rabbit anti-human GAPDH (Cell Signaling Technology, Beverly, MA, USA), and rabbit anti-human phospho-AKT (Thr308) (Abcam, Cambridge, MA, USA), and then incubated overnight at 4 °C. ..

    Article Title: A Meroterpenoid from Tibetan Medicine Induces Lung Cancer Cells Apoptosis through ROS-Mediated Inactivation of the AKT Pathway.
    Article Snippet: An aliquot of the denatured supernatant containing 40 mg of protein was resolved on SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with a blocking buffer (Tris-buffered saline, i.e., TBS, containing 5% non-fat milk) for 1 h at room temperature, the membrane was probed with primary antibodies for mouse anti-human caspase-8 (BD Biosciences, San Jose, CA, USA), rabbit Molecules 2023, 28, 1939 12 of 15 anti-human caspase-3, rabbit anti-human poly (ADP-ribose) polymerase (PARP), rabbit antihuman phospho-AKT (Ser473), rabbit anti-human caspase-9, rabbit anti-human AKT, rabbit anti-human PDK-1, rabbit anti-human GAPDH (Cell Signaling Technology, Beverly, MA, USA), and rabbit anti-human phospho-AKT (Thr308) (Abcam, Cambridge, MA, USA), and then incubated overnight at 4 ◦C. ..

    Saline:

    Article Title: A Meroterpenoid from Tibetan Medicine Induces Lung Cancer Cells Apoptosis through ROS-Mediated Inactivation of the AKT Pathway
    Article Snippet: An aliquot of the denatured supernatant containing 40 mg of protein was resolved on SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with a blocking buffer (Tris-buffered saline, i.e., TBS, containing 5% non-fat milk) for 1 h at room temperature, the membrane was probed with primary antibodies for mouse anti-human caspase-8 (BD Biosciences, San Jose, CA, USA), rabbit anti-human caspase-3, rabbit anti-human poly (ADP-ribose) polymerase (PARP), rabbit anti-human phospho-AKT (Ser473), rabbit anti-human caspase-9, rabbit anti-human AKT, rabbit anti-human PDK-1, rabbit anti-human GAPDH (Cell Signaling Technology, Beverly, MA, USA), and rabbit anti-human phospho-AKT (Thr308) (Abcam, Cambridge, MA, USA), and then incubated overnight at 4 °C. ..

    Article Title: A Meroterpenoid from Tibetan Medicine Induces Lung Cancer Cells Apoptosis through ROS-Mediated Inactivation of the AKT Pathway.
    Article Snippet: An aliquot of the denatured supernatant containing 40 mg of protein was resolved on SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with a blocking buffer (Tris-buffered saline, i.e., TBS, containing 5% non-fat milk) for 1 h at room temperature, the membrane was probed with primary antibodies for mouse anti-human caspase-8 (BD Biosciences, San Jose, CA, USA), rabbit Molecules 2023, 28, 1939 12 of 15 anti-human caspase-3, rabbit anti-human poly (ADP-ribose) polymerase (PARP), rabbit antihuman phospho-AKT (Ser473), rabbit anti-human caspase-9, rabbit anti-human AKT, rabbit anti-human PDK-1, rabbit anti-human GAPDH (Cell Signaling Technology, Beverly, MA, USA), and rabbit anti-human phospho-AKT (Thr308) (Abcam, Cambridge, MA, USA), and then incubated overnight at 4 ◦C. ..

    Membrane:

    Article Title: A Meroterpenoid from Tibetan Medicine Induces Lung Cancer Cells Apoptosis through ROS-Mediated Inactivation of the AKT Pathway
    Article Snippet: An aliquot of the denatured supernatant containing 40 mg of protein was resolved on SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with a blocking buffer (Tris-buffered saline, i.e., TBS, containing 5% non-fat milk) for 1 h at room temperature, the membrane was probed with primary antibodies for mouse anti-human caspase-8 (BD Biosciences, San Jose, CA, USA), rabbit anti-human caspase-3, rabbit anti-human poly (ADP-ribose) polymerase (PARP), rabbit anti-human phospho-AKT (Ser473), rabbit anti-human caspase-9, rabbit anti-human AKT, rabbit anti-human PDK-1, rabbit anti-human GAPDH (Cell Signaling Technology, Beverly, MA, USA), and rabbit anti-human phospho-AKT (Thr308) (Abcam, Cambridge, MA, USA), and then incubated overnight at 4 °C. ..

    Article Title: A Meroterpenoid from Tibetan Medicine Induces Lung Cancer Cells Apoptosis through ROS-Mediated Inactivation of the AKT Pathway.
    Article Snippet: An aliquot of the denatured supernatant containing 40 mg of protein was resolved on SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with a blocking buffer (Tris-buffered saline, i.e., TBS, containing 5% non-fat milk) for 1 h at room temperature, the membrane was probed with primary antibodies for mouse anti-human caspase-8 (BD Biosciences, San Jose, CA, USA), rabbit Molecules 2023, 28, 1939 12 of 15 anti-human caspase-3, rabbit anti-human poly (ADP-ribose) polymerase (PARP), rabbit antihuman phospho-AKT (Ser473), rabbit anti-human caspase-9, rabbit anti-human AKT, rabbit anti-human PDK-1, rabbit anti-human GAPDH (Cell Signaling Technology, Beverly, MA, USA), and rabbit anti-human phospho-AKT (Thr308) (Abcam, Cambridge, MA, USA), and then incubated overnight at 4 ◦C. ..

    Incubation:

    Article Title: A Meroterpenoid from Tibetan Medicine Induces Lung Cancer Cells Apoptosis through ROS-Mediated Inactivation of the AKT Pathway
    Article Snippet: An aliquot of the denatured supernatant containing 40 mg of protein was resolved on SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with a blocking buffer (Tris-buffered saline, i.e., TBS, containing 5% non-fat milk) for 1 h at room temperature, the membrane was probed with primary antibodies for mouse anti-human caspase-8 (BD Biosciences, San Jose, CA, USA), rabbit anti-human caspase-3, rabbit anti-human poly (ADP-ribose) polymerase (PARP), rabbit anti-human phospho-AKT (Ser473), rabbit anti-human caspase-9, rabbit anti-human AKT, rabbit anti-human PDK-1, rabbit anti-human GAPDH (Cell Signaling Technology, Beverly, MA, USA), and rabbit anti-human phospho-AKT (Thr308) (Abcam, Cambridge, MA, USA), and then incubated overnight at 4 °C. ..

    Article Title: A Meroterpenoid from Tibetan Medicine Induces Lung Cancer Cells Apoptosis through ROS-Mediated Inactivation of the AKT Pathway.
    Article Snippet: An aliquot of the denatured supernatant containing 40 mg of protein was resolved on SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). .. After blocking with a blocking buffer (Tris-buffered saline, i.e., TBS, containing 5% non-fat milk) for 1 h at room temperature, the membrane was probed with primary antibodies for mouse anti-human caspase-8 (BD Biosciences, San Jose, CA, USA), rabbit Molecules 2023, 28, 1939 12 of 15 anti-human caspase-3, rabbit anti-human poly (ADP-ribose) polymerase (PARP), rabbit antihuman phospho-AKT (Ser473), rabbit anti-human caspase-9, rabbit anti-human AKT, rabbit anti-human PDK-1, rabbit anti-human GAPDH (Cell Signaling Technology, Beverly, MA, USA), and rabbit anti-human phospho-AKT (Thr308) (Abcam, Cambridge, MA, USA), and then incubated overnight at 4 ◦C. ..



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